Genetic polymorphisms of MASP2, TG5, and DQA1 genes in Holstein and Brown Swiss dairy cows

نویسندگان

چکیده

The objective of this study was to investigate polymorphisms MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, Brown Swiss dairy cows (35 each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR carried out amplification 305-bp 545-bp TG5, 373-bp genes. assessment elaborated nucleotide sequence variations forms single (SNPs) between two breeds. SNPs identified the investigated Holstein breed A46G MASP2 gene C371T gene. A characteristic T53C SNP also reported Chi-square revealed a significant variation distribution all (P < 0.001). Consequently, could be used candidates reference guide efficient characterization Holsteins, breeds; therefore, developing marker assisted selection (MAS) production immunity traits breeds cattle.

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ژورنال

عنوان ژورنال: Journal of the Hellenic Veterinary Medical Society

سال: 2023

ISSN: ['1792-2720', '2585-3724']

DOI: https://doi.org/10.12681/jhvms.29524